tgf β quantikine elisa kit  (R&D Systems)

Journal: Journal of Cellular Physiology

Article Title: Role of Chitinase 3–like‐1 in Myelofibrosis via Fibroblast‐Produced Extracellular Matrix Enhancement

doi: 10.1002/jcp.70166

Figure Lengend Snippet: Significant increase in CHI3L1 expression associated with fibrocyte differentiation. (A) Heatmap of RNA sequencing analysis of human monocytes and fibrocytes. The list of differentially expressed genes was further filtered for genes with an absolute FPKM value of > 1 in each group, and a heatmap was generated. After extracting 25 genes that exhibited high variability, a map was drawn. (B) Human fibrocyte culture assay. The ratio of spindle‐shaped cells increased with culture time. (C) Quantitative real‐time polymerase chain reaction (PCR) of human fibrocytes. CHI3L1 mRNA expression increased with fibrocyte differentiation. (D) Enzyme‐linked immunosorbent assay (ELISA) analysis of human fibrocytes. CHI3L1 protein levels correlated with the degree of mRNA expression. (E, F) ELISA analysis of human fibrocyte culture supernatants. The amount of CHI3L1 and TGF‐β1 produced by fibrocytes collected from patients positive for JAK2 V617F was comparable to that from healthy donors. * p < 0.05, ** p < 0.01.

Article Snippet: The human fibrocyte supernatant was evaluated using human CHI3L1 and TGF‐β Quantikine ELISA Kit (R&D systems), and mouse serum samples were measured using mouse CHI3L1 Quantikine ELISA Kit (R&D systems), following the manufacturer's instructions.

Techniques: Expressing, RNA Sequencing, Generated, Real-time Polymerase Chain Reaction, Enzyme-linked Immunosorbent Assay, Produced

Journal: Frontiers in Immunology

Article Title: Development and validation of a potency assay matrix for optimized and consistent manufacture of clinical mesenchymal stem/stromal cells

doi: 10.3389/fimmu.2026.1725191

Figure Lengend Snippet: MSC-derived EVs drive Treg polarization. (A) Representative flow cytometry plots and quantification of FoxP3 and CD25 expression in CD4 + T cells treated with different numbers of EVs or without EVs (Ctrl). Data are means ± SD. n = 4 technical replicates using one PBMC donors. (B) IL-10 levels in the supernatant of T cells incubating with a range of EV numbers or without EVs. Data are means ± SD. n = 3 technical replicates using one PBMC donors. (C) Replication index ratio of FoxP3-positive vs. FoxP3-negative CD4 + T cells treated with different numbers of EVs or without EVs. Data are means ± SD. n = 3 technical replicates using one PBMC donors. (D) Representative imaging flow cytometry images of CD4 + and CD8 + T cells incubated with CBO-labeled EVs for 24hrs. (E) ELISA TGFβ1 levels on EVs (4 x 10 11 EVs used for ELISA) from MSC-CM derived from three different MSC donors. Data are means ± SD. n = 3 technical replicates using three independent EVs preparations per MSC donor. (F) Frequency of FoxP3 + cells among CD4 + T cells treated with 4 x 10 11 EVs for 3 days in combination with 5µM SB431542 or 1µM A83–01 or vehicle control (DMSO) or incubated without EVs (Ctrl). Data are means ± SD. n = 3 technical replicates using one PBMC donors. (G) Frequency of FoxP3 + cells among CD4 + T cells treated with or without 4 x 10 11 EVs for 3 days ± 5µg/mL anti-TGFβ1 antibody or isotype control. Data are means ± SD. n ≥ 3 technical replicates using ≥ 3 different PMBC donors. Statistical significance was determined using one-way ANOVA with Tukey’s HSD test. *P ≤ 0.05; **P ≤ 0.01; ***P ≤ 0.001; ****P ≤ 0.0001.

Article Snippet: TGFβ1 concentration was evaluated using the TGFβ1 ELISA kit (R&D Systems, DB100C) following manufacturer’s instructions.

Techniques: Derivative Assay, Flow Cytometry, Expressing, Imaging, Incubation, Labeling, Enzyme-linked Immunosorbent Assay, Control